Press Releases

2020.11.25

Multicolor Activatable Raman Probes for Simultaneous Detection of Plural Enzyme Activities : Jingwen Shou (D2), Associate Professor Yasuyuki Ozeki, Department of Electrical Engineering and Information Systems, and other researchers.

Raman probes based on alkyne or nitrile tags hold promise for highly multiplexed imaging. However, sensing of enzyme activities with Raman probes is difficult because few mechanisms are available to modulate the vibrational response. Here we present a general strategy to prepare activatable Raman probes that show enhanced Raman signals due to electronic preresonance (EPR) upon reaction with enzymes under physiological conditions. We identified a xanthene derivative bearing a nitrile group at position 9 (9CN-JCP) as a suitable scaffold dye, and synthesized four types of activatable Raman probes, which are targeted to different enzymes (three aminopeptidases and a glycosidase) and tuned to different vibrational frequencies by isotope editing of the nitrile group. We validated the activation of the Raman signals of these probes by the target enzymes and succeeded in simultaneous imaging of the four enzyme activities in live cells. Different cell lines showed different patterns of these enzyme activities.



Journal of the American Chemical Society : https://pubs.acs.org/doi/10.1021/jacs.0c09200